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Biodiversity of Antimicrobial-Producing Actinomycetes Strains Isolated from Dry Dipterocarp Forest Soil in Northeast Thailand BABT
Chanthasena,Panjamaphon; Nantapong,Nawarat.
The increasing use of antibiotics has led to the development of drug-resistant microorganisms. The emergence of resistant strains has compromised the treatment and control of infectious diseases. As a result, the search and development of a novel class of antibiotic drugs are required. Actinomycetes have been recognized as a richest source of secondary metabolites including antibiotics. The aim of this study was to investigate the diversity of antibiotic-producing soil Actinomycetes from dry dipterocarp forest in Northeast of Thailand. The soil from this area has been known for its poor in nutrients and highly acidic with pH values around 4.5. In such a harsh condition, soil-inhabiting Actinomycetes elevate their protective mechanisms by inducing the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil bacteria; Antibiotics; Actinomycetes; Streptomyces.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100417
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Characterization of streptomycetes with potential to promote plant growth and biocontrol Scientia Agricola
Sousa,Carla da Silva; Soares,Ana Cristina Fermino; Garrido,Marlon da Silva.
Studies with streptomycetes in biocontrol programs and plant growth promotion are presented as technological alternatives for environmental sustainable production. This work has the objective of characterizing six isolates of streptomycetes aiming the production of extracellular enzymes, indole acetic acid, capacity for phosphate solubilization, root colonization and growth under different pH and salinity levels. For detection of enzyme activity the isolates were grown in culture media with the enzyme substrates as sole carbon source. The root colonization assay was performed on tomato seedlings grown on 0.6% water-agar medium. Growth under different pH and salinity levels was evaluated in AGS medium with 1%, 1.5%, 2%, 2.5%, and 3% NaCl, and pH levels...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; Indole acetic acid; Extracellular enzymes; Siderophores.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000100007
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Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea BJM
Sreevidya,M.; Gopalakrishnan,S.; Kudapa,H.; Varshney,R.K..
Abstract The main objective of the present study was to isolate and characterize actinomycetes for their plant growth-promotion in chickpea. A total of 89 actinomycetes were screened for their antagonism against fungal pathogens of chickpea by dual culture and metabolite production assays. Four most promising actinomycetes were evaluated for their physiological and plant growth-promotion properties under in vitro and in vivo conditions. All the isolates exhibited good growth at temperatures from 20 °C to 40 °C, pH range of 7–11 and NaCl concentrations up to 8%. These were also found highly tolerant to Bavistin, slightly tolerant to Thiram and Captan (except VAI-7 and VAI-40) but susceptible to Benlate and Ridomil at field application levels and were found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickpea; Actinomycetes; PGP; Streptomyces; Biocontrol; Gene expression.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100085
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Fibrinolytic protease production by new Streptomyces sp: DPUA 1576 from Amazon lichens Electron. J. Biotechnol.
M.M. Silva,Germana; Bezerra,Raquel P; Teixeira,José A; Porto,Tatiana S; Lima-Filho,José L; Porto,Ana Lúcia F.
Background Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 2² factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm² was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied...
Tipo: Journal article Palavras-chave: Actinomycetes; Factorial design; Glucose; Microbial enzymes; Soybean flour.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100004
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Growth and antioxidant response of juvenile oysters Crassostrea sikamea and Crassostrea corteziensis treated with Streptomyces strains Arq. Bras. Med. Vet. Zootec.
García-Bernal,M.; Medina-Marrero,R.; Campa-Córdova,Á.I.; Mazón-Suástegui,J.M..
ABSTRACT The effect of three Streptomyces strains (N7, RL8 and V4) and a mixture of Bacillus (BMix) on the growth (Weight, Size) and superoxide dismutase activity (SOD) in hatchery-reared juvenile oysters Crassostrea corteziensis and Crassostrea sikamea was investigated to determine their probiotic potential. Microorganisms were added to culture water at 1×106 CFU/ml once a day during 30 days and all oysters fed daily a microalgae mix. Juveniles of C. sikamea treated with strains N7, RL8 and V4 had a significant weight gain compared to the control group. C. corteziensis juveniles treated with strains RL8 and BMix showed a significantly higher weight gain than the control group. No significant size increase was observed in any treated group for both oyster...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; Oyster; Growth; Probiotic effect; SOD activity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601993
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In vitro antimicrobial activity and characterization of mangrove isolates of streptomycetes effective against bacteria and fungi of nosocomial origin BABT
Das,Arijit; Bhattacharya,Sourav; Mohammed,Abuelgasim Yegoup Hassan; Rajan,Subbaramiah Sundara.
The study aimed at determining the in vitro antimicrobial activity of alkaliphilic and halotolerant actinomycetes isolated from a mangrove ecosystem and identification of a potent strain. Twenty five isolates of actinomycetes were isolated from the sediment samples of Valapattanam mangrove swamp in Kerala, India. Antimicrobial activity of four selected actinomycete isolates was determined against bacterial and fungal pathogens of nosocomial origin by agar well diffusion method. Molecular characterization of the potent isolate was performed by 16S rDNA sequencing. Isolate no I-1 significantly inhibited Staphylococcus aureus ATCC 25923 (12 mm), S. aureus (15±0.05 mm), S. citreus (20±0.5 mm), Bacillus cereus (17±0.2 mm) and Serratia marcescens (12 mm). It...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces xiamenensis; Actinomycetes; Antimicrobial activity; Mangrove swamp.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300006
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Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.) BABT
Araújo,Janete M. de; Silva,Adilson C. da; Azevedo,João L..
Actinomycetes were isolated from surface sterilized leaves and roots of maize. A total of 53 isolates were obtained, 31 of them from leaves and 22 from roots. The genus Microbispora was the most frequently found followed by the genera Streptomyces and Streptosporangium. From the isolated actinomycetes, 43.4% showed antimicrobial activity against one or more tested bacteria and yeast.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytes; Maize; Actinomycetes; Microbispora; Streptomyces; Streptosporangium.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400016
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Population dynamics and extracellular enzymes activity of mesophilic and thermophilic bacteria isolated from semi-arid soil of Northeastern Brazil BJM
Gorlach-Lira,Krystyna; Coutinho,Henrique D.M..
The dynamics of mesophilic and thermophilic bacterial population was studied in the bulk soil and rhizosphere of the grass Aristida adscensionis L. in a caatinga of the semi-arid Brazilian Northeast. Mesophilic heterotrophic bacteria, sporeforming bacteria and actinomycetes were significantly more abundant than thermophiles, and their counts were 10(6) - 10(7) CFU g-1 dry soil, showing weak fluctuations over the one-year study period. Thermophiles were below 10(6) CFU g-1 dry soil and, in general, demonstrated stronger fluctuations over the studied period. Among them, thermophilic actinomycetes showed the greatest decline during the dry season. No significant differences in microbial number were observed between bulk and rhizosphere soil. Mesophilic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesophilic bacteria; Thermophilic bacteria; Actinomycetes; Extracellular enzymes; Semi-arid; Caatinga.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100028
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Production of streptomycete inoculum in sterilized rice Scientia Agricola
Soares,Ana Cristina Fermino; Sousa,Carla da Silva; Garrido,Marlon da Silva; Perez,Jane Oliveira.
Actinomycetes are important plant disease control and growth promotion agents, which makes it necessary to develop technology to produce large quantities of inoculum for green-house and field work. The present study had the objective of evaluating the growth of several isolates of Streptomyces in sterile rice for inoculum production. The sterile rice was inoculated with isolates of S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and Streptomyces sp., and incubated at 28 ± 2ºC. Five days after its inoculation, mycelial growth and sporulation was observed for all Streptomyces isolates on the rice grains. Twelve days after incubation, the colonized rice was transferred to envelopes of dark brown paper and let to dry in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Actinomycetes; Biological control; Growth substrates.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600013
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Screening of actinomycetes from earthworm castings for their antimicrobial activity and industrial enzymes BJM
Kumar,Vijay; Bharti,Alpana; Negi,Yogesh Kumar; Gusain,Omprakash; Pandey,Piyush; Bisht,Gajraj Singh.
Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%), Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Enzymes; Earthworm castings; Actinomycetes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100022
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Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens Acta Amazonica
SILVA,Germana Michelle de Medeiros e; BEZERRA,Raquel Pedrosa; TEIXEIRA,José António; SILVA,Flávio Oliveira; CORREIA,Juliana Mendes; PORTO,Tatiana Souza; LIMA-FILHO,José Luis; PORTO,Ana Lúcia Figueiredo.
ABSTRACT Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm2. Three variables at two levels were used to assess their effects on the fibrinolytic production. The parameters studied were agitation (0.28 - 1.12 g), temperature (28 - 36 ºC) and pH (6.0 - 8.0); all of them had significant effects on the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; Fibrinolytic protease; Inhibitor protease; Fibrinogenolytic activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000300323
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Seasonal variation of microbial population from sediments of Vellar Estuary, South India. ArchiMer
Lakshmanaperumalsamy, P; Chandramohan, D; Natarajan, R.
A seasonal study on the occurrence and distribution of total aerobic microbial population (TMP), total aerobic heterotrophic bacteria (THB), actinomycetes and fungi in the sediments of Vellar Estuary, South India (11 degree 29'N 79 degree 46'E) was carried out during Jan. to Dec. 1975. Sediment samples were collected at monthly intervals, from 4 stations and analysed microbiologically. In general, bacterial population was found to be dominant followed by actinomycetes and fungi. The max peak for TMP, THB and fungi were recorded in Nov. in all stations. Monsoon season supported TMP, THB and fungi to a high level and it was summer for actinomycetes. Statistical analysis (Kendall's tau) showed a significantly positive correlation among TMP, THB, actinomycetes...
Tipo: Text Palavras-chave: Actinomycetes; Population number; Statistical analysis; Seasonal variations; Fungi; Bacteria; Sediment analysis; Microbiological analysis.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-943.pdf
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Soil streptomycetes with in vitro activity against the yam pathogens Curvularia eragrostides and Colletotrichum gloeosporioides BJM
Soares,Ana Cristina Fermino; Sousa,Carla da Silva; Garrido,Marlon da Silva; Perez,Jane Oliveira; Almeida,Nailson Santos de.
Yam (Dioscorea cayennensis Lam), an important crop in the Northeastern region of Brazil, is susceptible to the attack of several phytopathogenic fungi, such as Curvularia eragrostides (Henn.) Meyer and Colletotrichum gloeosporioides (Penz.), which cause leaf spot diseases. This work aimed to evaluate the effect of six actinomycete isolates (S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and two isolates identified as Streptomyces sp.), as well as the effect of their secondary metabolites, on mycelium growth and spore germination of C. eragrostides and C. gloeosporioides, from yam plants, cultivar da Costa. Additionally, the chitinolytic activity of these actinomycete strains was determined in a minimum salt growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Dioscorea cayennensis; Actinomycetes; Secondary metabolites.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400010
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Streptomycetes antagonism against Cladosporium fulvum Cooke and Fusarium oxysporium f.sp. lycopersici Ciência Rural
Soares,Ana Cristina Fermino; Sousa,Carla da Silva; Garrido,Marlon da Silva.
This research aimed to evaluate the secondary effects of secondary metabolites produced by streptomycetes on spore germination and mycelial growth of the phytopathogenic fungi Cladosporium fulvum Cooke and Fusarium oxysporium f. sp. lycopersici from tomato plants. Metabolites produced by streptomycete isolates codified as AC-147 and AC-92 caused 94.1% inhibition of C. fulvum while AC-95 isolate caused 33.9% inhibition. AC-92 was the most efficient for F. oxysporum f. sp. lycopersici, causing 94.2% inhibition of spore germination. For mycelial growth, AC-26 and AC-92 were the most efficient in inhibiting C. fulvum growth by 46.6% and F. oxysporum f. sp. lycopersici by 29.9%. These streptomycetes are potential agents for biocontrol development methods of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Actinomycetes; Secondary metabolites.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600042
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The function of lexical motifs in the organization of the Actinomycetes 5S rRNAs BJM
Rodrigues-Subacius,Sandra M; Padilla,Gabriel.
This work shows results obtained by employing the linguistic method to identify biologically meaningful sites in Actinomycetes 5S rRNAs. The approach adopted identifies triplet-words, along the base sequence of 5S rRNA, located mainly at the alpha and beta domains of the 5S secondary structure. There are triplet-words representing universal protein binding sites that include important prokaryote signatures, and sites strategically located in critical regions related to the formation of the 5S ribonucleoproteins (RNP) complex. In those sites, where the GC pressure promoted substitutions, the analysis demonstrates that alterations did not affect their biological significance. Sites formed by GGY (or more rarely GGR), continued to play an important role as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; 5S rRNA; GC pressure; Linguistic analysis; Lexical motifs.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300036
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วิธีการใช้ Streptomyces-PR87 ในการส่งเสริมการเจริญเติบโตของมะเขือเทศ Thai Agricultural
Assiri Klangsawad; Petcharat Thummabenjapone.
Using plant growth promoting microbes is an alternative approach for reducing cost and enhancing good health of crop plants. This research aims to establish the application protocol for enhance growth of tomato at seedling and transplanting stages by antagonistic Streptomyces-PR87 which will be applied further to farmer. The experiments were carried out using two varieties of tomato, e.g. var.Sida and var.Tomato32. In seedling stage, the experiments were carried out in combinations with Streptomyces-PR87 concentration at OD600=0.2, 1 and 5 and drenching interval at 3, 5 or 7 days, and seed soaking for 24 hours before sowing or directly drenching after seed sowing methods. The results showed that the most appropriated method for enhance growth and...
Tipo: PhysicalObject Palavras-chave: Organic tomato; Microorganism; Biofertilizer; Actinomycetes; Growth promote; Bioregulators; Plant disease control; Germination; Streptomyces-PR87; มะเขือเทศ; เชื้อจุลินทรีย์; การส่งเสริมการเจริญเติบโต; ปุ๋ยชีวภาพ; แอคติโนมัยซีส; เชื้อแบคทีเรียปฏิปักษ์; สารควบคุมการเจริญเติบโต; การควบคุมโรค; การงอก; ความยาวของราก; ความสูงของลำต้น; การปลูกพืชอินทรีย์.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5785
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ศักยภาพของเชื้อ Streptomyces-PR87 ปฏิปักษ์ และวิธีการใช้สำหรับควบคุมไส้เดือนฝอยรากปม (Meloidogyne incognita) ในสภาพโรงเรือนปลูกพืชทดลอง Thai Agricultural
Rattikan Yutthasin; Petcharat Thummabenjapone; Anan Hiransalee.
The root-knot nematode (Meloidogyne incognita) caused root knot disease of several economic crops. This research aims to evaluate the potential of antagonistic Streptomyces-PR87 to control root knot nematode under laboratory condition and determines the suitable application to tomato root knot disease on under green house condition. The results showed that the culture filtrate of Streptomyces-PR87 obtained from 7 days old culture in Arginine glycerol mineral salt broth at concentration of 50, 75 and 100% significantly inhibited egg hatching of M. incognita at average of the number of second-stage juvenile (J2) larvae per 5 egg masses with 37.67, 6.67 and 3.33 larvae of J2 per 5 egg masses, respectively compared of 136.33 J2 from 5 egg masses in distilled...
Tipo: PhysicalObject Palavras-chave: Tomato; Tomato nematode; Root-knot nematode; Actinomycetes; Streptomyces-PR87; Microorganism; Biocontrol; Plant disease control; Pest control methods; มะเขือเทศ; ไส้เดือนฝอยรากปม; โรครากปม; แอคติโนมัยซีส; เชื้อจุลินทรีย์; เชื้อแบคทีเรียปฏิปักษ์; การควบคุมศัตรูพืช; การควบคุมโรค; การควบคุมโดยชีววิธี.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5786
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